Characterization of Bacillus thuringiensis from Cotton Fields and its Effectiveness against Spodoptera litura

Cotton is a cash crop of many countries as it serves source fiber, edible oil, and seedcake. It grown in diverse range environment. Bacillus thuringiensis (Bt) gram positive bacterium found different habitats but mostly soil. This produces endotoxin which harmful for various insects. Numerous crystal-forming strains Bt exist nature, not all them are efficient against insect pests. cotton prevalent internationally because less attack. The key objective this research was to analyze isolates residing soil fields Multan Bahawalpur districts identify on the basis morphology. To attain this, samples were collected from (Gup wala, Mosey Wala, Taloki Hafiz Choudary Basti Mangla Mari, Sharkha Lawain Ghulamo Wala) district (Basti Deewan Wali, Check No 8, Tariqabad, Khaji Khandin, Rammo Sultanpur, Khurampur, Jahanpur). About 178 colonies bacteria eighteen appeared Nutrient Agar plates. screening based morphology yielded 18 Bt. We performed endo-toxin production experiment confirmed presence parasporal crystals identified B. colonies. Colony forming units represented statistically significant results. Based morphology, eight out Results current study revealed that isolates, 8 bacterial media had an increased elevation, round, white, slimy smooth edges. Toxicity test resistance leaf worm second instar larvae two (BtS2, BtS7) toxic showed 39.25% mortality. will help local can be used indigenous pest control.